Caenorhabditis elegans egg-laying circuit consists of simple motor program with two classes of motor neurons, HSN (Hermaphrodite Specific Neuron) and VC (ventral cord neuron), vulva muscles and uterine muscles. I am studying how the activity of motor neurons, is controlled by intracellular signaling network consisting G protein and Phosphatidylinositol system (PI) . The study is based on powerful genetic editing tools such as CRISPR-Cas system integrated with Auxin-degron system, and optogenetics which allow us visualize expressed proteins; lipids and ions with live imaging techniques in the transgenic worms. The detail egg-laying circuit is being investigated creating transgenes in which major pathways of PI (4,5) P2 synthesis is modulated in individual cells to understand how the circuit provides information for neurotransmitter release and behavior of whole organism.